Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENVFDYEDIQLIPAKCIVNSRSECDTSVRLG-GHTFKLPVVPANMQTIIDEKLAISLAENGYFYVMHRFE-PE---TRIDFIKD------------MNARG---LFSSISVGVKDEEYEFVRQLAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAA---SKPIIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHEESPGQTIEKDGKLYKEYFGSASEFQK--GEKKNVEGKKMHVAHKGSIKDTLIEMEQDLQSSISYAGGTKLNAIRN-VDYVIVKNSIFNGDKY 4R7J Chain:A ((7-367)) VKRALTFEDVLLRPGYSEVL-PKEVKIHTKLTKNITLNMPLISAAMDTVTEHRAAIMMARLGGLGVIHKNMDIASQVREVKRVKKSEKKRKEYPDANKDNFGRLRVGAAI--GV--GQMDRVDALVEAG--VDVVVLDSAHGHSKGIIDTVKAIKAKYPNLDLIAGNIATAAAAKALCEAGVDAVKVGIGPGSICTTRIVSGVGVP--QISAIDECVEEANKFGVPVIADGGIKYSGDIAKALAVGASSVMIGSLLAGTDESPGELFTYQGRQYKSYRGMGSLGAMQK---LVPEGIEGRVPYVGSIRSVVHQLLGGLRSSMGYVGAKDIEDFQKRAEFVEITTAGLKESH-

General information: TITO was launched using: RESULT: Template: 4R7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1928 10912 5.66 34.86 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 5.66 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4R7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R7J-query.scw PDB file : Tito_Scwrl_4R7J.pdb: