Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCN-GDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP 4EK4 Chain:A ((4-293)) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKI---------PSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKP

General information: TITO was launched using: RESULT: Template: 4EK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 52248 for 2221 contacts (23.5/contact) + 2D Compatibility (PS) -28597 + (NN) -1060 + (LL) 240 1D Compatibility (HY) 20400 + (ID) 800 Total energy: 42431.0 ( 19.10 by residue) QMean score : 0.015

(partial model without unconserved sides chains): PDB file : Tito_4EK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EK4-query.scw PDB file : Tito_Scwrl_4EK4.pdb: