Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVITEQNTYLNFINGEWVKSQSGDMVKVENPADVNDIVGYVQNSTAEDVERAVTAANEAKT--AWRKLTGAERGQYLYKTADIMEQRLEEIAACATREMGKTLPEAK-GETARGIAILRYYAGEGMRKTGDVIP-----STDKDALMFTTRVPLGVVGVISPWNFPVAIPIWKMAPALVYGNTVVIKPATETAVTCAKIIACFEEAGLPAGVINLVTGPGSVVGQGLAEHDGVNAVTFTGSNQVGKIIGQAALARGAKYQLEMGGKNPVIVADDADLEAAAEAVITGAFRSTGQKCTATSRVIVQSGIYERFKEKLLQRTKDITIGDSLKEDVWMGPIASKNQLDNCLSYIEKGKQEGASLLIGGEKLENGKYQNGYYVQPAIFDNVTSEMTIAQEEIFGPVIALIKVDSIEEALNIANDVKFGLSASIFTENIGRMLSFIDEIDAGLVRINAESAGVELQAPFGGMKQSSSHSREQGEAAKDFFTAIKTVFVKP 4O5H Chain:A ((28-509)) ------AKPKQMLIGAEWSDAASGRQLDVVNPAD-GTVIARVPEADERDVQQAVAAARRAFDAGPWRTAKTTDRERLMLVLADLIEANARELAEIESLDNGKPVMVAQGLDVAMAAQCFRYMAGWATKIEGSVIDAGMPYLPDSEIFAYTRKEPVGVVGAIIPWNFPLLMAAWKIAPALATGCTVVLKPAEDTPLSALRLGELIQAAGFPDGVVNIVTGYGHTAGAALSRDPRIDKIAFTGSTQTGKTIGHAALDNMTRMSLELGGKSPVIVLPDVDLDKAAQGVANAIFFNQGQVCTAGSRAYIHSKVFDGVIERVAKIAASLKIGPGMDPATQIGPLVSAKQRERVCGYIDSGFGEGARAAAGGRAI----DGPGFFVEPTVLVDTTQAMRVVREEIFGPVLVAMPFDDVDTAVQLANDTPYGLGASIWSNDLSAIHKLVPRIAAGTVWVNC-HSLLDNALPFGGMKQSGFG-RELGRAVIDQYTESKSVMMN-

General information: TITO was launched using: RESULT: Template: 4O5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3012 -234357 -77.81 -494.42 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -77.81 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4O5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O5H-query.scw PDB file : Tito_Scwrl_4O5H.pdb: