Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTGHFSML-DTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCTKEG-GVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELKADLLRICGTDFGLTD-PSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTKLID-FTQAGLHLRSMMSELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARLPDMKLILSDGNSERLYSFLQNGTFVLLSLRQEADDH-I--EVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPDCSEVIQKGISRWFSVTSRV 4K5S Chain:A ((13-510)) -ALTTDVVVVGGGPVGLMLAGELRAGGVGALVLEKLVEPVGHDRAGALHIRTVETLDLRGLLDRFLEGTQVAKGLPFAGIFTQGLDFGLVDTRHPYTALVPQSRTEALLAEHAREAGAEIRRGHEVTGLRQDAEAVEVTVAGP-SGPYRVRARYAVGCDGGRSTVRRLAGIGFPGTEATVRALIGYVTTPEREVPR-RWERTPDGILVLAFPPEGGLGRVVVIEYT------EGPVTLEDLGAAVARVRGTPLTLTEPVSWLSRFGDASRQAKRYRSGRVLLAGDAAHVHFPIGGQGLNTGLQDAVNLGWKLAARVRGWGSEELLDTYHDERHPVAERVLLNTRAQLALMRPDEQHTTPLRGFVEELLGTDEVNRYFTGMITGTDVRYATFAP-RPHPWAGRFAGGLVLSGPSGEPVPVAELLRSARPLLLDLAGRADLREATRPWSDRVSVVA--G-EATVEPPAQALLVRPDGYVAWAGSPAAT-ADELRA-SLARWFGP----

General information: TITO was launched using: RESULT: Template: 4K5S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2879 -254552 -88.42 -530.32 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -88.42 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_4K5S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K5S-query.scw PDB file : Tito_Scwrl_4K5S.pdb: