Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKIGVSLIV------LIMLIIFLAGCNKNEQNGDETKMQSLVGYVVLKDNE-----------RAILITDTKAPGKEDYNLSEGQLM----------NKFKNNIVIVGLSEIDNTDDLKRGEKIKVW--------FHTRKESNPPSATIQKYELL 1W4S Chain:A ((26-171)) MYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGE-----KWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVYVCESRYSAKT--KSFKKIKLWTMPVSSVRFVPRDVPLPVVRVASVFA--

General information: TITO was launched using: RESULT: Template: 1W4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 450 19493 43.32 175.61 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 43.32 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_1W4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W4S-query.scw PDB file : Tito_Scwrl_1W4S.pdb: