Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGYLHDAFSLKGKTALVTGPGTGIGQGIAKALAGAGADIIGTSHT-SSLSETQQLVEQEGRIFTSFTLDMSKPEAIKDSAAELFE-NRQIDILVNNAGIIHREKAEDFPEENWQHVLNVNLNSLFILTQLAGRHMLKR-GHGKIINIASLLSFQGGILVPAYTASKHAVAGLTKSFANEWAASGIQVNAIAPGYISTANTKPIRDDEKRNEDILKRIPAGRWGQADDIGGTAVFLASRASDYVNGHILAVDGGWLSR 4G81 Chain:D ((3-253)) -----ALFDLTGKTALVTGSARGLGFAYAEGLAAAGARVILNDIRATLLAESVDTLTRKGYDAHGVAFDVTDELAIEAAFSKLDAEGIHVDILINNAGIQYRKPMVELELENWQKVIDTNLTSAFLVSRSAAKRMIARNSGGKIINIGSLTSQAARPTVAPYTAAKGGIKMLTCSMAAEWAQFNIQTNAIGPGYILTDMNTALIEDKQFDSWVKSSTPSQRWGRPEELIGTAIFLSSKASDYINGQIIYVDGGWLA-

General information: TITO was launched using: RESULT: Template: 4G81.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1335 -30123 -22.56 -121.46 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.81 3D Compatibility (PKB) : -22.56 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_4G81.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G81-query.scw PDB file : Tito_Scwrl_4G81.pdb: