Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFPKKLLPIAVLSSIAFSSLASGSVPEASAQEKKKGNQDEIKNVIVLIGDGMGVSYTSAYRYLKDNKKT-KVVEPTAFDQYLVGQQ-TTYPDDPEQNVTDSAAAATAMSAGIKTYNNAIAVDNDG------------SEAKTVLEAAKEKGKATGLVATSEITHATPASFGSHDHSRKNM---------------NSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKD---------------RNLIKEFK------KAGYSYVDDRKDMLKN---KDSQVLGLFADGGLPKKIDRT-KDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQIDWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVATADHSTGGYSIGADGIYNWFSEPIK-AAKRTPDFMAEKIADGADVEKTLKTYIDQK--------KLALTKAEIQSVEEAAKSKEVLDIDNAIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKALQYNIKINDK 1K7H Chain:A ((23-472)) -------------------------------------LREKQAKNVIFFLGDGMSLSTVTAARIYKGGLTGKFEREKISWEEFDFAALSKTYNT--DKQVTDSAASATAYLTGVKTNQGVIGLDANTVRTNCSYQLDESLFTYSIAHWFQEAGRSTGVVTSTRVTHATPAGTYAHVADRDWENDSDVVHDREDPEICDDIAEQLVFREP--GKNFKVIMGGGRRGFFPEEALDIEDGIPGEREDGKHLITDWLDDKASQGATASYVWNRDDLLAVDIANTDYLMGLFSYTHLDTVLTRDAEMDPTLPEMTKVAIEMLTKDENGFFLLVEGGRIDHMHHANQIRQSLAETLDMEEAVSMALSMTD-PEETIILVTADHGHTLTITGYADRN---T-DILDFAGISDLDDR--------RYTILDYGSGPGYHITEDGKRYEPTEEDLKDI------------------NFRYASAAPKHSATHDGTDVGIWVNGPFAHLFTGVYEENYIPHALAYAACVGTGR---

General information: TITO was launched using: RESULT: Template: 1K7H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2596 -124742 -48.05 -322.33 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -48.05 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_1K7H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K7H-query.scw PDB file : Tito_Scwrl_1K7H.pdb: