Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGIHTLT-QFEAVFLGAVGNPKLV-PDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLHPNDFDFVVIRENSEGEYSEVGGRIHRG-DDEIAIQNAVFTRKATERVMRFAFELAKKR------------R-SHVTSATKSNGIYHAMPFWDEVFQQTA---ADYSGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDHFGEEELGAKILDVMEQVTADGI-KTRDIGGQSTTAEVTDEICSRLRKL 3TY4 Chain:A ((9-364)) ---RRIVLGLIPADGIGKEVVPAARRLMENLPAKH-KLKFDFIDLDAGWGTFERTGKALPERTVERLKTECNAALFGAVQSPTHKVAGYSS---PIVALRKKMGLYANVRPVKSLDGAKG-----KPVDLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIAKSRQKIRESGTYSIHKKPLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSYASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANVGDN---FVMSEPVHGSAPDIAGRGIANPVATFRSVALMLEFMGHQDAAADIYTAVDKVLTEGKVLTPDLGGKSGTNEITDAVLANI---

General information: TITO was launched using: RESULT: Template: 3TY4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1937 -177356 -91.56 -527.85 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -91.56 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3TY4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TY4-query.scw PDB file : Tito_Scwrl_3TY4.pdb: