Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYGGKSAEHNVSLQTALAVIKALNTEKFDIHPIYITEKGEWVRGARLTEPVSNVKMLQFEQGGSAFSPAALNQEMFPQEASQQNEAIDVVFPLLHGPNGEDGTIQGMLELLNIPYVGNGVLASSAGMDKVVMKHLFAQVGLAQAKYAAFLKKDWTRSPKESCEEVEQELGYPCFVKPANLGSSVGISKCRNREELQKAFELAFQYDRKVVVEEGING-REIEIGVLGND-DPKCSVVGEIAPKTDFYDYKAKYEDGDTDLMIPAIVTDEEYATISDMAIKAFKAIDGSGLVRADFFLTADGEVLINEVNTMPGFTPFSMFPLLWKEAGVEYADLIEQLVELAKERHAEKQLIKHTF 4EG0 Chain:A ((19-315)) LFGGESAEREVSLTSGRLVLQGLRDAGIDAHPFDPAERP--------------LS----------------------AL---KDEGFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGVPTPPFETVMRGDDYAA---RATDIVAKLGLPLFVKPAS-----AVLKVKTADALPAALSEAATHDKIVIVEKSIEGGGEYTACIAGDLDLPLIK----IVP---------------TQYLIPCGLPAEQETELKRIARRAFDVLGCTDWGRADFMLDAAGNAYFLEVNTAPGMTDHSLPPKAARSIGIGYSELVVKVLSLTL-------------

General information: TITO was launched using: RESULT: Template: 4EG0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1472 -139966 -95.09 -508.97 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -95.09 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4EG0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EG0-query.scw PDB file : Tito_Scwrl_4EG0.pdb: