Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------MNGIAWMIVFCEIAFWVVIVLGLAVRYVFKRHTLGLLF-----LALTPVI--DLILLAAT-------GVDLYRGASATAAHGIAAVYIGISIAYGKQMIQWADEKF----QYYV----TKKGTKPLKRFG----------------MDHAKHGAKGWLRHVLAYLI----------GAGLLAGMIYFINDSSRTEALSGI-LKLWTVIIGIDFLIT-ASYFIWPKKEKASANLRS 4QCC Chain:A ((5-274)) TKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQWEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGMTVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPENLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAQKQKEQRQDQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTLQAFEA

General information: TITO was launched using: RESULT: Template: 4QCC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 480 -84845 -176.76 -458.62 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -176.76 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.139

(partial model without unconserved sides chains): PDB file : Tito_4QCC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QCC-query.scw PDB file : Tito_Scwrl_4QCC.pdb: