Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDIRKIERLAAASWPAYFQKSIGKWLLRANFGVTKRANSVWTSADMPEGDFQLEAELFYQSLGL-------PVCFHISNASPKGLDDALADSRYEKVDECFQMTALCRSIMSRTNDNSRFTYKWEQEPS----SVWIDEFIQLEGF-SPERHKGYKHIFE--RMPPCKTFFKMYD-KESLTALGTVSVIDGYGGLSNIVVAEEHRGKGAGTQVIRVLTEWAKNNGAERMFLQVMK-ENLAAVSLYGKIGFSPISEHHYRIKR 3TT2 Chain:A ((100-315)) -----------------------------------------------LGTWLVQWGEEWIQDRMHLAPAEAQVTVQHYIRASSTSALRLMEQHGYRPVRDIWVMAITLDQPPPAP--EWPEGITARTFVPGLDERATYEAVEEAFG-RPPSTFERWLSMTQSERKDPELWLLAVETDSGHIVGTCLGQETAGKGWIGSVGVRRPWRGRGIALALLQEVFGVYYRRGVREVELSVDAESRTGAPRLYRRAGMHVKHRYVLHRK-

General information: TITO was launched using: RESULT: Template: 3TT2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 746 24485 32.82 124.92 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 32.82 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_3TT2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TT2-query.scw PDB file : Tito_Scwrl_3TT2.pdb: