Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEKKERIIKTSIRLFAKKGFAATTIQEIASECGISKGAFYLHFKSKEALLLSACEYYI-GMSMNKMKNIEEDL--AGKPPKEVL-KKQIGAQFEDFRDHKDFIVLLLTENIIPENQEIKQYFYKVTMETDKLYRNALLVSYGEGIERYVADLSIMARGIVHSYMNVMVFNGELNIDAEEISAFIIERLDDLVQGLSRSALNPIVSKDIFNPMPAGKDQLLEDIQKVKENSTLPEDITVSL-DVIEEELTQDKPRKPIIKGMLSNLA-GTNDKEVER-LRALILS-LSQFDHKKSSL 1ZKG Chain:A ((13-211)) VLSKRDAILKAAVEVFGKKGYDRATTDEIAEKAGVAKGLIFHYFKNKEELYYQA----YMSVTEKLQKEFENFLMKNRNRDIFDFMERWIEKKLEYSASHPEEADFLITLVSV--DEGLRKRILLDLEKSQRVFFDFVREKLKDLDLA---------------------------------------------------------------------------------EDVTEEIALKFLMWFFSGFEEVYLRTYQGKPELLKR-DMNTLVEEVKVMLRILKKGMTK---------

General information: TITO was launched using: RESULT: Template: 1ZKG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 622 -61199 -98.39 -320.41 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -98.39 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_1ZKG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZKG-query.scw PDB file : Tito_Scwrl_1ZKG.pdb: