Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYGDSREKILSAATRLFQLQGYYGTGLNQIIKESGAPKGSLYYHFPGGKEQLAIEAVNEMKEYIRQKIADCMEA-CTDPAEGIQAFLKELSCQFSCTEDIEGLPVGLLAAETSLKSEPLREACHEAYKEWASVYEEKLRQTGCSESRAKEASTVVNAMIEGGILLSLTAKNSTPLLHISSCIPDLLKR 3ON4 Chain:A ((7-186)) -NISNTKERILAVAEALIQKDGYNAFSFKDIATAINIKTASIHYHFP-SKEDLGVAVISWHTDKIAAVLSDISNNSSLSAKEKIQKFFDAILTLTYNSE-NKMCLGGMFASDFQSLPVSIQNQAKKFFELIIEWLKGVLETNGYDNESSLSLAKQIISLVEGGLLLARLYGDETFLEGVRHFI------

General information: TITO was launched using: RESULT: Template: 3ON4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 695 -93958 -135.19 -524.91 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -135.19 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_3ON4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ON4-query.scw PDB file : Tito_Scwrl_3ON4.pdb: