Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADLKTQILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLILARTAKDIKYNADYIKRHLKEVKQMPQDVYEGYLSKTEEFQKNDLHLLESDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYDHIHRILEEEGLLENGSFDISVMVAFGYRVRDPRPKTRSAVEDVVKWV 1NOX Chain:A ((6-205)) ---PVLDAKTAALKRRSIRRYRK-DPVPEGLLREILEAALRAPSAWNLQPWRIVVVRDPATKRALREAAFG-QAHVEEAPVVLVLYADLEDALAHLDEVIHP------GVQGERREAQKQAIQRAFA----A-MGQEARKAWASGQSYILLGYLLLLLEAYGLGSVPMLGFDPERVRAILG----LP-SRAAIPALVALGYPAEEGYPSHRLPLERVVLWR

General information: TITO was launched using: RESULT: Template: 1NOX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 823 -111770 -135.81 -558.85 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -135.81 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_1NOX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NOX-query.scw PDB file : Tito_Scwrl_1NOX.pdb: