Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGYSMVQMVRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFEEGCHETGFQMAGEVLQKEKRSKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNREVKALLDTATEASHAKEVVRNGQ-TLTGKGVTVAVVDTGI-YPHPDLEGRIIGFADMVNQ-----KTEPYDDNGHGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCIQYNEDN-------------PDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQTIASPGVSEKVITVGALDDNNTASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSPNSYIDKLQKSSRVGSQYFTMSGTSMATPICAGIAALILQQN-------------PDLTPDEVKELLKNGTDKWKD-EDPNIYGAGAVNAENSVPGQ 2Z30 Chain:A ((8-317)) ----------------------------------------------------------------------------------------------------------------------------IPWGIERVKAPSV--WSITDGSVSVIQVAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQNGHGTHVIGTIAALNN--DIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIEQAILGPDGVADKDGDGIIAGDPDDDAAEVISMSLGGPADD------SYLYDMIIQAYNAGIVIVAASGNEGAP--SPSYPAAYPEVIAVGAIDSND-------NIASFSNRQ--------PEVSAPGVDILSTYP--------------DDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKILPVGTFDDISKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQA-

General information: TITO was launched using: RESULT: Template: 2Z30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1940 -191244 -98.58 -692.91 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -98.58 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2Z30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z30-query.scw PDB file : Tito_Scwrl_2Z30.pdb: