Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEKRSSTGFLVKQRAFLKLYMITMTEQERLYGLKLLEVLRSEFKEIGFKPNHTEVYRSLHELLDDGILKQIKVKKEGAKLQEVVLYQFKD--YEAAKLYKKQLKVELDRCKKLIEKALSDNF 3HHH Chain:A ((11-104)) ---------------KGILEGLVLAIIQRKETYGYEITKI---LNDQGFTEIVEGTVYTILLRLEKNQWVIAEKK--PSE--PMRKFYRLTSSGEAELADFWQRWTLLSKQVNK----------

General information: TITO was launched using: RESULT: Template: 3HHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 293 -35409 -120.85 -393.43 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -120.85 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_3HHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HHH-query.scw PDB file : Tito_Scwrl_3HHH.pdb: