Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGTVNITVSDGVRYAVADEGPNASEAVVCLHGFTGSKQSWT-FLD-EMLPDSRLIKIDCLGHGETDAPLNGKRYSTTRQVSDLAEIFDQLKLHKVKLIGYSMGGRLAYSFAMTYPERVSALVLESTTPGLKTLGERRERIMRDRKLADFILRDGLEAFVAYWENIPLFSSQQRLAEDIRYRIRSG---RLRNNKIGLANSLTGMGTGSQPSLWSRVEEIDVPVLLICGEWDEKFC-AINQEVHKMLPSSRIEIVPKAGHTVHVEQPRLFGKIVSEFLTSI 4PW0 Chain:A ((21-283)) --------VNGTRYAYRSLGAPSDIPLICFQHFTGTLDNWDPLITNGLSKGRQLIIFDNKGVGLSSGTT---PDNVAAMTADALEFITALGIRYFDVLGFSLGGFIVQYMAHIQPDMIRKIIIVGAAPQGVKVLHTFPDLIA--RAMQL---EPKERFLFIFFEQSEHSRSKGLATLG-RLYERTTDRDQDASAQAIGAQLTAITNWGKKTPSFEITSIQHPVFVVQGSNDEMMDTYNSYELFKQLPDAILSLYPDAAHGSFYQYPELFVSQTEYFLDSY

General information: TITO was launched using: RESULT: Template: 4PW0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1470 -14545 -9.89 -56.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -9.89 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_4PW0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PW0-query.scw PDB file : Tito_Scwrl_4PW0.pdb: