Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQQYIVEPKKGLGLKLKKGQILKVVDVEGQQVADFVAYHAKDFYEHLDQGATIDANHSIHVKVNDHLYSNLYKPMLTLIEDTV-GKHDLLLPACRPDMNRLLYGKQKDE-FQDTCYDNMNRALEQFGVPKPHMHYPFAIFMNTVLDEKGNLSVETPLSNAGDYVRLRAEMDLIVAFSSCPIEKGKCNGDSVTSIRVEVS 3SIY Chain:A ((37-227)) ----YTIPARQGRAIRMAQGEALMVINRDGSQIGDFWAFVEGDCGEYLSMEHLRPTLRRVSPRPGDVLVSNRRRPILTLLEDSSPGVHDTLVASC--DVHR--YAQLGHEGYHDNCTDNLRMALGALGLRPTTVPCPLNLWMNTPVVEGGAMEWRPPVSRRGDHVLFRAELDVVVVISCCPMDLLPINGEEAQPRALDV-

General information: TITO was launched using: RESULT: Template: 3SIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1095 -111729 -102.04 -591.16 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -102.04 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_3SIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SIY-query.scw PDB file : Tito_Scwrl_3SIY.pdb: