Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFALLFIALVTAVVISACGNQSTSSKGSDTKKEQITVKHQLDKNGT-KVPKNPKKVVVFDFGSLDTLDKLGLDDI-VA--GLPKQVLPKYLSKFKDDKYADVGSLKEPDFDKVAELDPDLIII-SARQSESYKEFSKIAPTIYLGVDTAKYMESFKSDAETIGKIFDKEDKVKDELANIDHSIADVKKTAEKLNKNGLVIMANDGKISAFG-PKSRY--GLIHDV-FGVAPADQNIKASTHGQSVSY-----EYISKTNPDYLFVIDRGTAIGETSSTKQVVENDYVKNVNAVKNGHVIYLDSATWYLSGGGLESMTQMIKEVKDGLEK 3LHS Chain:A ((8-287)) ------------------------------------ISVK---DENGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGD--YTSVGTRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINS-FKTIAKALNKEKEGEKRLAEHDKLINKYKDEI-KFDRNQKVLPAVVAKAGLLAHPNYSYVGQFLNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIM-TDHAKKDSAEFKKLQEDATWKKLNAVKNNRVDIVDRDVWARS-RGLISSEEMAKEL------

General information: TITO was launched using: RESULT: Template: 3LHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1376 -125281 -91.05 -470.98 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -91.05 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_3LHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LHS-query.scw PDB file : Tito_Scwrl_3LHS.pdb: