Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINVDVKQNENDIQVNIAGEIDVYSAPVLREKLVPLAEQGADLRIC--LKDVSYMDSTGLGVFVGTFKMVKKQGGSLKLENLSERLIRLFDITGLKDIIDISAKSEGGVQ 1H4X Chain:A ((15-95)) ---------------IRLFGELDHHAVEQIRAKISTAIFQGAVTTIIWNFERLSFMDSSGVGLVLGRMRELEAVAGRTILLNPSPTMRKVFQFSGL---------------

General information: TITO was launched using: RESULT: Template: 1H4X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 266 -52588 -197.70 -665.66 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -197.70 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.701

(partial model without unconserved sides chains): PDB file : Tito_1H4X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H4X-query.scw PDB file : Tito_Scwrl_1H4X.pdb: