Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNKELQKLTEDISETYFKKPFRHQ---ALFNDRLKTTGGRYLLTSHNIELN-RKYLIEHGREELIG--IIKHEL--CHYHLHLEGKGYKHRDRDFRMLLQQVNAPRFCTPLKKKAENKKTYMYICTTCGQQYIKKRAMNPDRYRCGKCRGKIKRIFS 1BYG Chain:A ((62-161)) ------------LAEASVMTQLRHSNLVQLLGVIVEE--GLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVL----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1BYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 149 -19078 -128.04 -244.58 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -128.04 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.133

(partial model without unconserved sides chains): PDB file : Tito_1BYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BYG-query.scw PDB file : Tito_Scwrl_1BYG.pdb: