Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTPKKHKKFKAKMILQIIMVIIGGIIAAYGLETVLIPNSVSDGGVTGLSIVGSQLFNLPLGILIAVINIPFVWLGYKQIGKSFALLSIIGIVSLAAGTSFFHHTPAIIEGDTLLITVVGGIILGFGMGLALRNGGALDGIDMLAVLLSRKLPFGTSDLILFLNLFVFIFVSTVFGLQGALLSVIAYYIASKVIHVVEEGLSGSKTFQIITTQ-PELMVETIRDQLGRSATYKEAYGGFSHEKFKEITCVINRLEETKLKEIINDIDKTAFVTVYDVAEVKGSNFRNLNHH 4RLE Chain:A ((12-74)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IVAVVQDQDSNRLLKTLTD-HNFRVTKLATTGGFLKSGNTTFMIGVEDIRVNKALSLIKENGQK----------------------

General information: TITO was launched using: RESULT: Template: 4RLE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 196 -29852 -152.31 -481.48 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.43 3D Compatibility (PKB) : -152.31 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_4RLE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RLE-query.scw PDB file : Tito_Scwrl_4RLE.pdb: