Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIIIPNNEIAKHMLTDFFAKHWGTPEMAISSGIFRCDELD-----GFAAVNESGEIIGCI----TYTIDGKDCEIISLDSMIENKGIGTALLQQVEEKAKHAHCQRIKLIT-----------------TNDNVNAIA--------FYQKRGYQFAAVFPNAVEKARRLKPEIPEVAENGILIRDEILFSKVID 1N71 Chain:A ((9-161)) ------NNPVLKDQLSDLLRLTW--PEEYGDSSAEEVEEMMNPERIAVAAVDQD-ELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNA--------------------------------

General information: TITO was launched using: RESULT: Template: 1N71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 388 -64009 -164.97 -537.89 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -164.97 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_1N71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N71-query.scw PDB file : Tito_Scwrl_1N71.pdb: