Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRMKVNQMSLLEHIAELRKRLLIVALAFVVFFIAGFFLAKPIIVYLQETDEAKQLTLNAFNLTDPLYVFMQFAFIIGIVLTSPVILYQLWAFVSPGLYEKERKVTLSYIPVSILLFLAGLSFSYYILFPFVVDFMKRISQDLNVNQVIGINEYFHFLLQLTIPFGLLFQMPVILMFLTRLGIVT-PMFLAKIRKYAYFTLL-VIAALITPP-----ELLSHMMVTVPLLILYEISILISKAAYRKAQKSSAADRDVSSGQ 1DAF Chain:A ((138-206)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLPVILVVGVKLGCINHAMLTAQVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWL-----------------------

General information: TITO was launched using: RESULT: Template: 1DAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 184 -54913 -298.44 -885.69 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -298.44 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_1DAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DAF-query.scw PDB file : Tito_Scwrl_1DAF.pdb: