Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRIILLLAVIIAAAAAGVAFYVAKDKGHEKAADVSVNTESGDELLVSITDTDLLTKYYENDKVIHEEKLTSYPAFALDQKQQVLYYTGNNE-----QNEMRLFKLDLKSHKKTMLYKGAESADSLFLSKDRSTIYFRLGKADESNFRIAAFDLKTKKYKNLYPAANDQD-----------------DTVSSFFYNQKNDSFALLHYSVEEDYKKTDE--ANEK-----------GIDPEPTTIHFAEGR--QNKFN---------ELKSLNQFISDIAVSDDDKRILFTSYTQKGTEQTASIQMLNADT-------KKYESIISNQKSFKLLI-----DAQPQFSKDGKNIYF-LAEAKGAKKLKDETGREAKVRTIYSYSLENKTFKKVWENPNGIINSFFVIN 1Z68 Chain:A ((63-353)) --------------------------------------------------------------------------SNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVY------QNNIYLKQRPGDPPFQITFN--GRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDI-PVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQN---VSVLSICDFREDWQTWDCPKTQEHIEESRT---GWAGGFFVSTPVFSYDAISYYKIFSDKDGY-------------KHIHYIKDTVENAIQIT--------------

General information: TITO was launched using: RESULT: Template: 1Z68.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 985 -74986 -76.13 -323.22 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -76.13 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_1Z68.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z68-query.scw PDB file : Tito_Scwrl_1Z68.pdb: