Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITPFLDKKSKTPLYEQLYTFFKQEISHARITKGTRLPSKRRLSSLLDVSTATIERAYEQLTAEGYVKSKPKIGWFAAEVEPGFPTAPDHFQQSVQPGLHQKNAPAIDFHQGSVDPTLFPFNAWRKSIVKSLDRYSGSFHTSGDP-QGEYELRHMIARFVRLSRGVNCEPGQIIIGAGTTVLLQNLC-LSLKPGTKIGFEEPGFHRSRRMFEANHMDVTPICSDAEG----VLPDELYRQNPYLMYTTPSHQFPIGTIMTITRRQELLAWAAETNSFIIEDDYDGEFRYSGHPIPSLQGLDPNGR---VIYLGTFSKSLLPSLRLSFMIVPPELMEPIQNNVQLMKQTVSAHSQLALADFIETGEWQKHINRMRSLYRKKHAVLLEAIRSELGNTVEILGKNSGLHILLRLLFPASEEEAIQAAADHGVTLYPVSPSYIEQTPFTSVLIGYGGLSEEDIRLGIQKLKTAWAPLISSY 2ZP7 Chain:C ((35-395)) -----------------------------------------------------------------------------------------------------------LSFAGGLPAPELFPKEEAAEAAARIL-REKGEVALQYSPTEGYAPLRAFVAEWI------GVRPEEVLITTGSQQALDLVGKVFLDEGSPVLLEAPSYMGAIQAFRLQGPRFLTVPAGEEGPDLDALEEVLKRERPRFLYLIPSFQNPTGGLTPLPARKRLLQMVMERGLVVVEDDAYRELYFGEARLPSLFELAREAGYPGVIYLGSFSKVLSPGLRVAFAVAHPEALQKLVQAKQGADLHTPMLNQMLVHELLKEG-FSERLERVRRVYREKAQAMLHALDREVPKEVRYTRPKGGMFVWMELPKGLSAEGLFRRALEENVAFVPGGPFFANGGGENTLRLSYATLDREGIAEGVRRLGRALKGLLA--

General information: TITO was launched using: RESULT: Template: 2ZP7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1987 -235870 -118.71 -670.09 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -118.71 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2ZP7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZP7-query.scw PDB file : Tito_Scwrl_2ZP7.pdb: