Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVSNVTVRKRLLFVLLFGVIVFLIIDTRLGYVQFVMGEKLTSLAKDS----WSRNLPFEPERGEILDRNGVKLATNKSAPTVFVVPRQVQNPM-------------KTSKQLAAVLNMSEEKVYKHVTKKASIEKIT--PEGRKISNEKA----KEIKALDLKGVYVAEDSIRHYPFGSFLSHVLGFAGIDNQ--------GLLGLEAYYDDDLKGEKGSVKFYTDAKGKKMPDEADDYTPPKDGLDMKLTVDSKVQTIMERELDNAEAKYHPDGMIAVAMNPKNGEILGMSSRPDFDPADYQSVDPSVYNRNLPVWSTYEPGSTFKIITLAAALEEQKVNLKRDQFYDKGHAEVDGARLRCWKRG----GHGLQTYLEVVQNSCNPGFVELGERLGKEKLFKYIKDFGFGQKTGIDLQGEGTGILFPLERVGPVEQATTAFGQGVSVTPIQQVAAVSAAVNGGTLYTPYIAKEWIDPVTKKTVKKQSPIAKKQVISEETSKQIRYALE--------SVVAEGTGRNAF-VEGYRVGGKTGTAQKV--KDGKYL--ENNHIVSFIGFAPADDPSLVVYVAVDNPK--GTIQFGGTVAAPIV--GNIMRDSLPELGVKPRKNQIEKKYQWNDTKTIEVPNVVGMSVSDL-ESLLVNL-NVDASGKGSKIVKQSPAAGTKVKEGSKIRVYLTEEDEKEAAD 1RP5 Chain:A ((16-647)) ------------------------------------------------KVHQTTRTVP--AKRGTIYDRNGVPIAEDATSYNVYAVIDENYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQ-PNLKQVSFGAKGNGITYANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSGMESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSPLQSFMETQMDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYEPGSTMKVMMLAAAIDNN--TFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQL-PADNI--VNIAMSAFGQGISVTQTQMLRAFTAIANDGVMLEPKFISALYDPNDQSVRKSQKEIVGNPVSKEAASVTRDHMVMVGTDPTYGTMYNHSTGKATVNVPGQNVALKSGTAEIADEKNGGYLTGSTNNIFSVVSMHPAENPDFILYVTVQQPEHYSGIQL-GEFANPILERASAMKESLNLQTTAKALEQVSQQSPY------PMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAPNQQVLILSDKAEE-----

General information: TITO was launched using: RESULT: Template: 1RP5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3283 -99030 -30.16 -171.33 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -30.16 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_1RP5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RP5-query.scw PDB file : Tito_Scwrl_1RP5.pdb: