Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFIKRLSASCESTIHHKVYQIMNEAKTEFEKVLKKLK 2IBF Chain:B ((2-22)) -----------------HAIYEKAKEVSSALSKVLSKI-

General information: TITO was launched using: RESULT: Template: 2IBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3 348 115.83 16.55 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 115.83 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_2IBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IBF-query.scw PDB file : Tito_Scwrl_2IBF.pdb: