Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPTKQHKKETFTHFIFRVFMILVGAASAAVSIELFLIPNDFIDGGIIGVSLILDHLFMSNPFLNFAFFVVILNIPFMIFGYKYIGKTFLVSTFIGIVGLAVIESSLHHVEAITTQPILATVFGGLLLGFGVGLVIRNGGSMDGTEILGILLTKKLPFSVGEFVMFINVFIFIWAVFVFGPEQAMYSFMTYYIAMKTIDAVIQGLDETKAVIIVSEQ--YDEISDAILHRLGRGTTKLKGKGG-YT---DEEKDVIYAVVTRLEVTKLKSIVFEVDQ-----NAFITIMNTHETRGGKFKNAIH 2O66 Chain:A ((14-114)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YKVEAIVRPWRIQQVSSALLKIGIRGVTVSDVRGFG--EDKFVAKVKMEIVVKKDQVESVINTIIEGARTGEIGDGKIFVLPVSDVIR--------

General information: TITO was launched using: RESULT: Template: 2O66.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 278 9510 34.21 126.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.43 3D Compatibility (PKB) : 34.21 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_2O66.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O66-query.scw PDB file : Tito_Scwrl_2O66.pdb: