Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANNNSGNSNNLLVPGAAQAIDQMKLEIASEFGVNLGADTTSRANGSVGGEITKRLVSFAQQNMGGGQF 1AVO Chain:A ((7-25)) -------------QPEAQAKVDVFREDLCTKT-------------------------------------

General information: TITO was launched using: RESULT: Template: 1AVO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4 527 131.75 27.74 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 131.75 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.671

(partial model without unconserved sides chains): PDB file : Tito_1AVO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AVO-query.scw PDB file : Tito_Scwrl_1AVO.pdb: