Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAVFIQGAEEHPAAFCYQVNGSCPRTVHTLGIQLVIYLACAAGMLIIVLGNVFVAFAVSYFKALHTPTNFLLLSLALADMFLGLLVLPLSTIRSVESCWFFGDFLCRLHTYLDTLFCLTSIFHLCFISIDRHCAICDPLLYPSKFTVRVALRYILAGWGVPAAYTSLFLYTDVVETRLSQWLEEMPCVGSCQLLLNKFWGWLNFPL-FFVPCLIMISLYVKIFVVATRQAQQITTLSKSLAGAA--KHERKAAKTLGIAVGIYLLCWLPFTIDTMVDSLLHFITPPLVFDIFIWFAYFNSACNPIIYVFSYQWFRKALKLTLSQKVFSPQTRTVDLYQE 3ZPQ Chain:A ((22-300)) --------------------------------------------VLLIVAGNVLVIAAIGSTQRLQTLTNLFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAIDRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSFLPIMMHWWRDEDPQALKCYQDPGCCDFVTNRAYAIASSIISFYIPLLIMIFVALRVYREAKEQ------------SRVMLMREHKALKTLGIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVAFNWLGYANSAMNPIIYCRSPD-FRKAFKRLLA----------------

General information: TITO was launched using: RESULT: Template: 3ZPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -252312 for 1986 contacts (-127.0/contact) + 2D Compatibility (PS) -26748 + (NN) -5059 + (LL) 6248 1D Compatibility (HY) -28400 + (ID) 5050 Total energy: -311321.0 ( -156.76 by residue) QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_3ZPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZPQ-query.scw PDB file : Tito_Scwrl_3ZPQ.pdb: