Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNDQIVFEKTKNIAHDINQMQNQQEIIDYLFRQDSLTLNQLKHYYSEPSLPLQFLVKVAVLCMFISMTLASFLFIQAKEVFTNTILSDISPAVFSIFTVICIFMTYTKIIKKGNKNKGKASLNQRSEFYEKNKLINTILYKKYKMDQQNIQANKHTASDNEDSMNFSAVLNHVLTISKNDKELLGYLDTRDNAMLSQLKAYFSTRPFSLPHYMSLMFCGSIIVVYATSLFSGQINYIDIPHIFIFLLLIIFLKILIDLIKLLNISRKGQLHTVLHFAQRAEYLRMRGVIDFILTERYNKKIM 2JN4 Chain:A ((1-24)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKVM-IRKTATGHSAYVAKKDLEEL-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2JN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 46 -3156 -68.61 -131.50 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -68.61 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.710

(partial model without unconserved sides chains): PDB file : Tito_2JN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JN4-query.scw PDB file : Tito_Scwrl_2JN4.pdb: