Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIDLQKKSVKIVLEKKQLTKVTARVGLVLDITGSMRPLYKN-------GTVQNVVERILAVADQFDDNGLLDVWVYDNEFSRLKPVSEK--DFSGYVDREILNNDRLHKFGRNDEPPVMKDVLRKYVTEEPSSYPAFIVFINDGGCKK---SIKPIIEASSDKPVFWQFVGIGNG-------------------NFDFLNKLDTLEGRVIDNTNFLHIEEIDRISDDELYDALLAEFPFWLKEAKEKGIVREQEPPAEKPKKKGFFSRLFSK 4RCK Chain:A ((17-209)) ----------------------EHRDMMLVVDLSGSMAEEDMKTSNGDFVDRLTAVKQVVSDFID-QRKGDRLGLVLFGDHAYLQTPLTFDRNTVREQLDRTVL----NLVGQRTAIGEGLGLATKTFIES--NAPQRTIILLSDGANTAGVLEPLEAAQLAKDNHAKIYTVGIGAGEMQVRGFFGKQTVNTARDLDEDTLTKIATM-----TGGQYFRARNADELA-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RCK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 611 -56754 -92.89 -350.33 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -92.89 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_4RCK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RCK-query.scw PDB file : Tito_Scwrl_4RCK.pdb: