Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFLHHILSTYASFFDWKMWGEVLTDPVSWGLIGSLVVLEGLLSADNALVLAVMVKHLPEKQRKKALTYGLFGAYIFRFIFIGLGMLLIKFWWIKVLGALYLAWLVIKHFWIGEKEEEADGMKKNSWMVRTFGIFWATVISVELMDLAFSVDSILAAFAVSEKVWVLLIGGMLGILMMRTVAKVFLVLIDKIPELENTAFVLIGIIALKMAGSAFHYEMPHSVFFIIIIAAFAVTLIIHYINKQKQVREQTAASKEE 1W24 Chain:A ((1-182)) ---------------------------------MLVLVLGDLHIPHRCNSLPAKFKKLLVPGK------------IQHILCTGN---LCTKESYDYLKTLAGDVHIVR----GDFDENLNYPEQKVVTVGQFKIGLIH------GHQVIPDMASLALLQRQFDVDILISGH---------------THKFEAFEHENKFYINPGSATGAYNALE---TNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKP-

General information: TITO was launched using: RESULT: Template: 1W24.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1037 -182843 -176.32 -1015.79 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -176.32 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_1W24.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W24-query.scw PDB file : Tito_Scwrl_1W24.pdb: