Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTKPFYRDTWAEIDLSAIKENVSNMKKHIGEHVHLMAVVKANAYGHGDAETAKAALDAGASCLAVAILDEAISLRKKGLKAPILVLGAVPPEYVAIAAEYDVTLTGYSVEWLQ---EAARHTKKGSLHFHLKVDTGMNRLGVK-TEEEVQNVMAILDRNPRLKCKGVFTHFATADEKERGYFLMQFERFKELIAPLP---LKNLMVHCANSAAGLRLKKGFFNAVRFGIGMYGLRPSADMSDEIPFQLRPAFTLHSTLSHVKLIRKGESVSYGAEYTAEKDTWIGTVPVGYADGWLRKLKGTDILVKGKRLKIAGRICMDQFMVELDQ--EYPPGTKVTLIGRQGDEYISMDEIAGRLETINYEVACTISSRVPRMFLENGSIMEVRNPLLQVNISN 4LUY Chain:A ((8-385)) ---------TWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYGHGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKITMTVYSLETAQKINEIAKSLGEKAC-VHV-IDSGMTRIGFQPNEESVQEIIE-LNKLEYIDLEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLRLNMVRAGIILYGHYPSDDVFKD-RLELRPAMKLKSKIGHIKQVEPGVGISYGLTYTTTGKETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEVILFG-EGE--VTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK-----

General information: TITO was launched using: RESULT: Template: 4LUY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2065 -261697 -126.73 -711.13 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -126.73 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_4LUY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LUY-query.scw PDB file : Tito_Scwrl_4LUY.pdb: