Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MADVLRRA-INQKKQFLKTKLLLSEFYQG---RGEQLADYTLSELEKEYKSLQKMKKEI------------------------------ 1JQE Chain:A ((13-292)) GKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKISNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA

General information: TITO was launched using: RESULT: Template: 1JQE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 98 -3942 -40.22 -71.66 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -40.22 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_1JQE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JQE-query.scw PDB file : Tito_Scwrl_1JQE.pdb: