Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTEDASRMEAMSLDYFLSYPRLFWPKFKE-LFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGARYDLPHLLER----EVPECTAAGNNGDICGTVLKTDVVLFGDAVMHFDTLYEKL---DQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 3D4B Chain:A ((3-239)) MKEFLDLLNESRLTVTLTGAGISTPSGIPDF------------NV--FDIDFFYSHPEEFYRFAKEGIFPMLQA---KPNLAHVLLAKLEEKGLIEAVITQNIDRLHQRAGSKKVIELHGNVEEYYCVRCEKKYTVEDVIKKLESSDVPLC-------DDCNSLIRPNIVFFGENLPQ-DALREAIGLSSRASLMIVLGSSLVVYPAAELPL-ITVRSGGKLVIVNLGETPFDDIATLKYNMDVVEFARRV----

General information: TITO was launched using: RESULT: Template: 3D4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1160 -145848 -125.73 -672.11 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -125.73 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_3D4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D4B-query.scw PDB file : Tito_Scwrl_3D4B.pdb: