Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLVNGRLIGRSEASIDLEDRGYQFGDGIYEVIRVY----KGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKLVQENAVSEG-----AVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKP-EQEQAYGVAAITDEDLRWLRCDIKSLNLLYNVMTKQRAYEAGAFEAILLRDGVVTEGTSSNVYAVINGTVRTHPANRLILNGITRMNILGLIEKNGIKLDETPVSEEELKQAEEIFISSTTAEIIPVVTLDGQSIGSGKPGPVTKQLQAAFQESIQQAASIS 4TM5 Chain:A ((20-295)) --------LVPLSEARVPVLDRGFIFGDGVYEVVPIYAEGARRAPFRIAQHLARLARSLKKIGIADPHDEAGWRALVARLVDANAAALGDGQHAIVYIQVTRGVAKRGHAFPANAVPTVFAMASPLALPTDAQRAQGVHCVTAEDRRWLHCDIKSVSLLGNVLMAQHAAEHDAAETIQLRDGNVTEGSSSNVWIVKNGELIAPPRSNRILEGIRYALVEELAEECGIRFVAREINEAELRAADEILLTSATKEILPVTRLDDLPVQGGRPGPVFDALYAAYQRA--------

General information: TITO was launched using: RESULT: Template: 4TM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1371 -119216 -86.96 -448.18 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -86.96 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4TM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TM5-query.scw PDB file : Tito_Scwrl_4TM5.pdb: