Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSGGYSNGFALLVVLFILLIIVGAAYIY--- 2MU8 Chain:A ((1-20)) KNESKYSNTF------------INNAYNMSIR

General information: TITO was launched using: RESULT: Template: 2MU8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 15 -1073 -71.53 -67.06 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -71.53 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_2MU8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MU8-query.scw PDB file : Tito_Scwrl_2MU8.pdb: