Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELHAITDDSKPVEELARII-ITIQNEVDFIHIRERSK---------SAADILKLLDLIFEGGIDKRKL-VMNGRVDIALFS---TIHRVQLPSGSFSPKQIRARFPHLHIGRSVHSLEEAVQAEKEDADYVLFGHVFETDCKKGLEGRGVSLLSDIKQRISI----PVIAIGGMTPDRLRDVKQAGADGIAVMSGIFSSAEPLEAARRYSRKLKEMRYEKAL 3O63 Chain:A ((32-239)) --LYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDELAACEILADAAHRYGALFAVNDRADIARAAGADVLHLGQRDLPVNVARQILA--PDTLIGRSTHDPDQVAAAAAGDADYFCVGPCWPTPT-----APGLGLV-----RVAAELDKPWFAIGGINAQRLPAVLDAGARRIVVVRAITSADDPRAAAEQLRSAL---------

General information: TITO was launched using: RESULT: Template: 3O63.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 956 -135246 -141.47 -743.11 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -141.47 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_3O63.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O63-query.scw PDB file : Tito_Scwrl_3O63.pdb: