Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALKAQNTISGKEGRLFLDGE-EMAHIKTFEANVEKNKSEVNIMGRRMTGHKTTGANGTGTATFYKVTSKFVLLMMDYVKKGSD-PYFTLQAVLDDQSSGRGTERVTLYDVNFDSAKIASLDVDSEALEEEVPFTFEDFDVPEKLSDTF 4TV4 Chain:A ((13-132)) ------------NGESLDDSHKDEIEVLNWNWEIQQ-------------------KASVKDLTFEHAIDRASPNLMKYALTGKHVDQAVLVMRKAGGN-PLEYLKLTMSDVIITRVRPSGSRDD--RSRETVSLSFAKV----------

General information: TITO was launched using: RESULT: Template: 4TV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 381 -32629 -85.64 -316.78 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -85.64 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4TV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TV4-query.scw PDB file : Tito_Scwrl_4TV4.pdb: