Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGA--MVYWTYEPTSLF-THWERYLIVAVSFALIDAFIFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIH-VYYGSIDAYAEGIDKLLKTYADKMNLTASLCHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVLPIEEEGEG 2PZS Chain:A ((389-511)) -------------------LYGKFASNPDVTGKVPYLKENGALGFRLGEEETKDPVYTPMGVFITAWARYTTITAAQACYDRIIYCDTDSIHLTGTEIPDVIKDIVDPKKLGYWAHESTFKRAKYLRQKTYIQDIYMKEVDG--------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2PZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 271 -27721 -102.29 -232.95 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -102.29 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.58 QMean score : -0.119

(partial model without unconserved sides chains): PDB file : Tito_2PZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PZS-query.scw PDB file : Tito_Scwrl_2PZS.pdb: