Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGMEWFPLLGLANRARKVVSGEDLVIKEIRNARAKLVLLTEDA-SSNTAKKVTDKCNYYKVPYKKVESRAVLGRSIGKEARVV-VAVTDQGFANKLISLLD 4C4W Chain:C ((18-105)) ----EALSLLEKVRESGKVKKGTNETTKAVERGLAKLVYIAEDVDPPEIVAHLPLLCEEKNVPYIYVKSKNDLGRAVGIEVPCASAAIINEG----------

General information: TITO was launched using: RESULT: Template: 4C4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 376 -50669 -134.76 -589.17 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -134.76 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_4C4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C4W-query.scw PDB file : Tito_Scwrl_4C4W.pdb: