Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHKVIVNHWEEICEDDSCYEYGTSIIVNGKELIREASIITALKAVLEEIGADVEIEETVESEKCCDSLRKKNLDY 1OBB Chain:A ((32-67)) ----------------------GSTVTLMDIDEERLDAILTIAKKYVEEVGADLKFEKTM----------------

General information: TITO was launched using: RESULT: Template: 1OBB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 70 -9149 -130.69 -254.13 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -130.69 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.614

(partial model without unconserved sides chains): PDB file : Tito_1OBB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OBB-query.scw PDB file : Tito_Scwrl_1OBB.pdb: