Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKFLRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPT-VHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIKQLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGF-VLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV 3E6E Chain:A ((7-370)) -----RPTRLHIDTQAITENVQKECQRLPEGTALFAVVKANGYGHGAVESAKAAKKGGATGFCVALLDEAIELREAGVQDPILILSVVDLAYVPLLIQYDLSVTVATQEWLEAALQQLTPE-SNTPLRVHLKVDTGMGRIGFLTPEETKQAVRFVQSHKEFLWEGIFTHFSTADEIDTSYFEKQAGRFKAVLAVLE----ELPRYVHVSNSATALWHPDVPGNMIRYGVAMYGLNPSGN-KLAPSYALKPALRLTSELIHVKRLAAG-EGIGYGETYVTEAEEWIGTVPIGYADGWLRHL--QGFTVLVNGKRCEIVGRVCMDQCMIRLAE--EVPVGPVVTLVGKDGNEENTLQMVAEKLETIHYEVACTFSQRIPREY----------------

General information: TITO was launched using: RESULT: Template: 3E6E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2077 -165354 -79.61 -456.78 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -79.61 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_3E6E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E6E-query.scw PDB file : Tito_Scwrl_3E6E.pdb: