Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTGYFLLEDGNKIEFELYPEAAPGTVANFEKLAN--EGF-YDGLTFHRVIPGFVSQGG-CPHGTGTGGPG-YTIKCETEGNPHTHEA-GALSMAHAGKDTGGSQFFIVHEPQPHLNGVHTVFGKVTSGLEFAKNM-----SNGDVMKEVRVEG 4DGD Chain:A ((19-158)) ------------RVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGNFTHCNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITI--

General information: TITO was launched using: RESULT: Template: 4DGD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 625 23302 37.28 180.63 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 37.28 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_4DGD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DGD-query.scw PDB file : Tito_Scwrl_4DGD.pdb: