Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLHGTSRQNQHGHLEIGGVDALYLAEKYGTPLYVYDVALIRERAKSFKQAFISAGLKAQVAYASKAFSSVAMIQLAEEEGLSLDVVSGGELYTAVAAGFPAER----IHFHGNNKSREELRMALEHRIGCIVVDNFYEIALLEDLCK-ETGHSIDVLLRITPGVEAHTHDYIT-TGQEDSKFGF---DLHNGQTERAIEQVLQSEHIQLLGVHCHIGSQIFDTAGFVLAAEKIFKKLDEWRDSYSFVSKVLNLGGGFGIRYTEDDEPLHATEYVEKIIEAVKENASRYGFDIPEIWIEPGRSLVGDAGTTLYTVGSQKEVPGVRQYVAVDGGMNDNIRPALYQAKYEAAAANRIGEA--HDKTVS--IAGKCCESGDML-------IWDIDLPEVKEGDLLAVFCTGAYGYSMANNYNRIPRPAVVFVENGEAHLVVKRETYEDIVKLDLPFKTGVKQ 1KNW Chain:A ((16-410)) --------------------NLLRLPAEFGCPVWVYDAQIIRRQIAALKQFDV-------VRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTADVIDQATLERVSELQIPV----NAGSVDMLDQLGQVSPGHR--VWLRVNPGF-GHGHSQKTNTGGENSKHGIWYTDL------PAALDVIQRHHLQLVGIHMHIGSGV-DYAHLEQVCGAMVRQVIEFGQDLQAISA----GGGLSVPYQQGEEAVD-TEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQSGVLITQVRSVKQM-GSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVETVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLLPEVLFDNGQARLIRRRQT-----------------

General information: TITO was launched using: RESULT: Template: 1KNW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2124 -17971 -8.46 -47.92 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -8.46 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_1KNW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KNW-query.scw PDB file : Tito_Scwrl_1KNW.pdb: