Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKFVEKMLGQALRQYGRNVAIDPLSPYEKQSLKAALQERRNEEPDEDLHAHIEDIIYDYVTNQGLFS 2LXE Chain:A ((63-102)) -DEKTMPVLMKLLEEAG--GNWSYIKLDNYTALVDAIYSVEDE-------------------------

General information: TITO was launched using: RESULT: Template: 2LXE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 74 -11888 -160.64 -297.19 target 2D structure prediction score : 0.93 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -160.64 2D Compatibility (Sec. Struct. Predict.) : 0.93 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_2LXE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LXE-query.scw PDB file : Tito_Scwrl_2LXE.pdb: