Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MQI-DIVQIVGLGLIA--TFLSL-IVKEQKPTFAF-LIVVFAGCAIFLYLVDQIYDIIRMIEKIAINANVNMVYVETILKIIGIAYIAEFGAQLTKDAGQGAIASKIELAGKILILVMAVPILT-VIIETILGLIPSMS 4DGW Chain:B ((4-143)) SRRDMEVIKLTARYYAKDKSIVEQMISKDGEARLNFMNSS-HPLHKTFTDFVAQYKRVYS--FTGQEIKKSKRTILDNCFERTQYWEFEKDKDREHDKLVELCKIQFAAIPWDKFTQVAKFDLEQMRLRR----------

General information: TITO was launched using: RESULT: Template: 4DGW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 142 -3211 -22.61 -26.75 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -22.61 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.227

(partial model without unconserved sides chains): PDB file : Tito_4DGW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DGW-query.scw PDB file : Tito_Scwrl_4DGW.pdb: