Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIGIFGGTFDPPHNGHLLMANEVLYQAGLDEIWFMPNQIPPHKQNEDYTDSFHRVEMLKLAIQSNPSFKLELVEMEREGPSYTFDTVSLLKQRYPNDQLFFIIGADMIEYLPKWYKLDELLNLIQFIGVKRPGFHVETPYPLLFADVPEFEVSSTMIRERFKSKKPTDYLIPDKVKKYVEENGLYES 4YMI Chain:A ((28-224)) -RRLGVMGGTFDPIHNGHLVAASEVADRFALDEVIFVPTGQP-----RKVSPAEHRYLMTVIATASNPRFTVSRADIDRGGATYTVDTLTDLRTAHPDADLYFITGADALASILSWENWEQLFTLAKFIGVSRPGY------GLSLVEVPALAISSTDCRIRAGQARPIWYLVPDGVVQYVAKHRLYSG

General information: TITO was launched using: RESULT: Template: 4YMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 913 -134714 -147.55 -761.09 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -147.55 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_4YMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YMI-query.scw PDB file : Tito_Scwrl_4YMI.pdb: